Structural characterization of a putative chitin synthase gene in Phytophthora spp. and analysis of its transcriptional activity during pathogenesis on potato and soybean plants.
Identifieur interne : 000918 ( Main/Exploration ); précédent : 000917; suivant : 000919Structural characterization of a putative chitin synthase gene in Phytophthora spp. and analysis of its transcriptional activity during pathogenesis on potato and soybean plants.
Auteurs : Lauren Hinkel [États-Unis] ; Manuel D. Ospina-Giraldo [États-Unis]Source :
- Current genetics [ 1432-0983 ] ; 2017.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (MeSH), Chitine synthase (génétique), Clonage moléculaire (MeSH), Maladies des plantes (microbiologie), Matrices de scores (MeSH), Motifs nucléotidiques (MeSH), Oomycetes (classification), Oomycetes (enzymologie), Oomycetes (génétique), Oomycetes (pathogénicité), Phylogenèse (MeSH), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Soja (microbiologie), Solanum tuberosum (microbiologie), Transcription génétique (MeSH).
- MESH :
- enzymologie : Oomycetes.
- génétique : Chitine synthase, Oomycetes.
- microbiologie : Maladies des plantes, Soja, Solanum tuberosum.
- pathogénicité : Oomycetes.
- Analyse de séquence d'ADN, Clonage moléculaire, Matrices de scores, Motifs nucléotidiques, Phylogenèse, Régions promotrices (génétique), Régulation de l'expression des gènes fongiques, Transcription génétique.
English descriptors
- KwdEn :
- Chitin Synthase (genetics), Cloning, Molecular (MeSH), Gene Expression Regulation, Fungal (MeSH), Nucleotide Motifs (MeSH), Oomycetes (classification), Oomycetes (enzymology), Oomycetes (genetics), Oomycetes (pathogenicity), Phylogeny (MeSH), Plant Diseases (microbiology), Position-Specific Scoring Matrices (MeSH), Promoter Regions, Genetic (MeSH), Sequence Analysis, DNA (MeSH), Solanum tuberosum (microbiology), Soybeans (microbiology), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : Chitin Synthase.
- classification : Oomycetes.
- enzymology : Oomycetes.
- genetics : Oomycetes.
- microbiology : Plant Diseases, Solanum tuberosum, Soybeans.
- pathogenicity : Oomycetes.
- Cloning, Molecular, Gene Expression Regulation, Fungal, Nucleotide Motifs, Phylogeny, Position-Specific Scoring Matrices, Promoter Regions, Genetic, Sequence Analysis, DNA, Transcription, Genetic.
Abstract
Although chitin is a major component of the fungal cell wall, in oomycetes (fungal-like organisms), this compound has only been found in very little amounts, mostly in the cell wall of members of the genera Achlya and Saprolegnia. In the oomycetes Phytophthora infestans and P. sojae the presence of chitin has not been demonstrated; however, the gene putatively encoding chitin synthase (CHS), the enzyme that synthesizes chitin, is present in their genomes. The evolutionary significance of the CHS gene in P. infestans and P. sojae genomes is not fully understood and, therefore, further studies are warranted. We have cloned and characterized the putative CHS genes from two Phytophthora spp. and multiple isolates of P. infestans and P. sojae and analyzed their phylogenetic relationships. We also conducted CHS inhibition assays and measured CHS transcriptional activity in Phytophthora spp. during infection of susceptible plants. Results of our investigations suggest that CHS contains all the motifs that are typical in CHS genes of fungal origin and is expressed, at least at the mRNA level, during in vitro and in planta growth. In infected tissues, the highest levels of expression occurred in the first 12 h post inoculation. In addition, results from our inhibition experiments appear to suggest that CHS activity is important for P. infestans normal vegetative growth. Because of the considerable variation in expression during infection when compared to basal expression observed in in vitro cultures of non-sporulating mycelium, we hypothesize that CHS may have a meaningful role in Phytophthora pathogenicity.
DOI: 10.1007/s00294-017-0687-6
PubMed: 28314907
Affiliations:
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In the oomycetes Phytophthora infestans and P. sojae the presence of chitin has not been demonstrated; however, the gene putatively encoding chitin synthase (CHS), the enzyme that synthesizes chitin, is present in their genomes. The evolutionary significance of the CHS gene in P. infestans and P. sojae genomes is not fully understood and, therefore, further studies are warranted. We have cloned and characterized the putative CHS genes from two Phytophthora spp. and multiple isolates of P. infestans and P. sojae and analyzed their phylogenetic relationships. We also conducted CHS inhibition assays and measured CHS transcriptional activity in Phytophthora spp. during infection of susceptible plants. Results of our investigations suggest that CHS contains all the motifs that are typical in CHS genes of fungal origin and is expressed, at least at the mRNA level, during in vitro and in planta growth. In infected tissues, the highest levels of expression occurred in the first 12 h post inoculation. In addition, results from our inhibition experiments appear to suggest that CHS activity is important for P. infestans normal vegetative growth. Because of the considerable variation in expression during infection when compared to basal expression observed in in vitro cultures of non-sporulating mycelium, we hypothesize that CHS may have a meaningful role in Phytophthora pathogenicity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28314907</PMID><DateCompleted><Year>2018</Year><Month>07</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-0983</ISSN><JournalIssue CitedMedium="Internet"><Volume>63</Volume><Issue>5</Issue><PubDate><Year>2017</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Current genetics</Title><ISOAbbreviation>Curr Genet</ISOAbbreviation></Journal><ArticleTitle>Structural characterization of a putative chitin synthase gene in Phytophthora spp. and analysis of its transcriptional activity during pathogenesis on potato and soybean plants.</ArticleTitle><Pagination><MedlinePgn>909-921</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00294-017-0687-6</ELocationID><Abstract><AbstractText>Although chitin is a major component of the fungal cell wall, in oomycetes (fungal-like organisms), this compound has only been found in very little amounts, mostly in the cell wall of members of the genera Achlya and Saprolegnia. In the oomycetes Phytophthora infestans and P. sojae the presence of chitin has not been demonstrated; however, the gene putatively encoding chitin synthase (CHS), the enzyme that synthesizes chitin, is present in their genomes. The evolutionary significance of the CHS gene in P. infestans and P. sojae genomes is not fully understood and, therefore, further studies are warranted. We have cloned and characterized the putative CHS genes from two Phytophthora spp. and multiple isolates of P. infestans and P. sojae and analyzed their phylogenetic relationships. We also conducted CHS inhibition assays and measured CHS transcriptional activity in Phytophthora spp. during infection of susceptible plants. Results of our investigations suggest that CHS contains all the motifs that are typical in CHS genes of fungal origin and is expressed, at least at the mRNA level, during in vitro and in planta growth. In infected tissues, the highest levels of expression occurred in the first 12 h post inoculation. In addition, results from our inhibition experiments appear to suggest that CHS activity is important for P. infestans normal vegetative growth. Because of the considerable variation in expression during infection when compared to basal expression observed in in vitro cultures of non-sporulating mycelium, we hypothesize that CHS may have a meaningful role in Phytophthora pathogenicity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hinkel</LastName><ForeName>Lauren</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Biology Department, Lafayette College, Easton, PA, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Cellular, Molecular, and Biomedical Sciences, University of Vermont, Burlington, VT, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ospina-Giraldo</LastName><ForeName>Manuel D</ForeName><Initials>MD</Initials><AffiliationInfo><Affiliation>Biology Department, Lafayette College, Easton, PA, USA. elsgenomics@gmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList 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MajorTopicYN="N">Position-Specific Scoring Matrices</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="Y">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Chitin</Keyword><Keyword MajorTopicYN="N">Chitin synthase</Keyword><Keyword MajorTopicYN="N">Oomycetes</Keyword><Keyword 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|area= PhytophthoraV1
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|clé= pubmed:28314907
|texte= Structural characterization of a putative chitin synthase gene in Phytophthora spp. and analysis of its transcriptional activity during pathogenesis on potato and soybean plants.
}}
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